Research Results – 東海大学における軟骨再生医療

Cartilage Regenerative Medicine at Tokai University

Research Results

2025

Auto- and Allo- Cartilage Cell Sheets for Osteoarthritis of the Knee

Masato Sato

August 14-15, 2025

16th annual meeting of the Asian Cell Therapy Organization (ACTO)

Regenerative Medicine for Osteoarthritis of the Knee Using Cell Sheets

Masato Sato, Eriko Toyoda, Kosuke Hamahashi

Regenerative Medicine in Sports and Orthopaedics, 2025

2024

International journal of Molecular Sciences, 25(18), 9981, 2024

Effect of Freeze-Thawing Treatment on Platelet-Rich Plasma Purified with Different Kits

Ryoka Uchiyama, Haruka Omura, Miki Maehara, Eriko Toyoda, Miyu Tamaki, Makoto Ogawa, Tatsumi Tanaka, Masahiko Watanabe and Masato Sato

2024

International journal of Molecular Sciences, 25(12), 6514, 2024

Disease-Modifying Effects of Lenvatinib, a Multiple Receptor Tyrosine Kinase Inhibitor, on Posttraumatic Osteoarthritis of the Knee

Yasuyuki Sogo, Eriko Toyoda, Toshihiro Nagai, Takumi Takahashi, Daichi Takizawa, Masahiko Watanabe, Masato Sato

2024

2023

Regenerative Therapy. Vol,24. December, 2023

Multiple intra-articular injections with adipose-derived stem cells for knee osteoarthritis cause severe arthritis with anti-histone H2B antibody production

Y.Hosono,A.Kuwasawa,E.Toyoda,K.Nihei,S.Sato,M.Watanabe,M.Sato

2023

2022

npj Regen Med, 7, 1, 2022.

Polydactyly-derived allogeneic chondrocyte cell-sheet transplantation with high tibial osteotomy as regenerative therapy for knee osteoarthritis

Hamahashi K, Toyoda E, Ishihara M, Mitani G, Takagaki T, Kaneshiro N, Maehara M, Takahashi T, Okada E, Watanabe A, Nakamura N, Kato R, Matoba R, Takagi T, Akutsu H, Umezawa A, Kobayashi H, Akamatsu T, Yamato M, Okano T, Watanabe M, and Sato M

2022

2021

npj Regen Med, 6, 65, 2021.

Safety and efficacy of human juvenile chondrocyte-derived cell sheets for osteochondral defect treatment

Kondo M, Kameishi S, Kyungsook Kim K, Nicolas F. Metzler, Travis G. Maak, Douglas T. Hutchinson, Angela A. Wang, Maehara M, Sato M, David W. Grainger & Okano T

2021

Int J Mol Sci, 22(6), 2021.

Development of Injectable Polydactyly- Derived Chondrocyte Sheets.

Wasai S, Toyoda E, Takahashi T, Maehara M, Okada E, Uchiyama R, Akamatsu T, Watanabe M, Sato M

2021

Int. J. Mol. Sci. 22(7), 2021.

Candidates for Intra-Articular Administration Therapeutics and Therapies of Osteoarthritis.

Toyoda E, Maehara M, Watanabe M, Sato M

2021

Int. J. Mol. Sci, 22(5), 2021.

Effect of Platelet-Rich Plasma on M1/M2 Macrophage Polarization.

Uchiyama R, Toyoda E, Maehara M, Wasai S, Omura H, Watanabe M, Sato M

2021

Int. J. Mol. Sci, 22(5), 2021.

Potential of Exosomes for Diagnosis and Treatment of Joint Disease: Towards a Point-of-Care Therapy for Osteoarthritis of the Knee.

Maehara M, Toyoda E, Takahashi T, Watanabe M, Sato M

2021

Arthritis Reseach & Therapy, 23.227, 2021.

Comparison of properties determined using electromechanical assessment (Arthro-BSTTM) with macroscopic and histological properties in symptomatic human articular cartilage of the hip.

Ukai T, Sato M, Wasai S, Takahashi T, Omura H, Watanabe M.

2021

2020

Regenerative Therapy, 14, 215-221, 2020.

Development of an efficient vitrification method for chondrocytesheets for clinical application.

Hayashi A, Maehara M, Uchikura A, Matsunari H, Matsumura K, Suong-Hyu H, Sato M

2020

J Tissue Eng Regen Med, [Epub ahead of print] 2020.

Regenerative effects of human chondrocyte sheets in a xenogeneic transplantation model using immune‐deficient rats.

Takizawa D, Sato M, Okada E, Takahashi T, Maehara M, Tominaga A, Sogo Y, Toyoda E, Watanabe M

2020

Scientific Reports, 10:10572, 2020.

Characteristics of autologous protein solution and leucocytepoor platelet-rich plasma for the treatment of osteoarthritis of the knee.

Wasai S, Sato M, Maehara M, Toyoda E, Uchiyama R, Takahashi T, Okada E, Iwasaki Y, Suzuki S, Watanabe M

2020

2019

International Journal of Moleculer Sciences, 21(1):E149, 2019.

Transcriptomic and Proteomic Analyses Reveal the Potential Mode of Action of Chondrocyte Sheets in Hyaline Cartilage Regeneration.

Toyoda E, Sato M, Takahashi T, Maehara M, Okada E, Wasai S, Iijima H, Nonaka K, Kawaguchi Y, Watanabe M

2019

NPJ Regen Med, 4, 4, 2019.

Combined surgery and chondrocyte cell-sheet transplantation improves clinical and structural outcomes in knee osteoarthritis.

Sato M, Yamato M, Mitani G, Takagaki T, Hamahashi K, Nakamura Y, Ishihara M, Matoba R, Kobayashi H, Okano T, Mochida J, Watanabe M

2019

Regenerative Therapy, 10, 17-26, 2019.

Multilineage-differentiating stress-enduring (Muse)-like cells exist in synovial tissue.

Toyoda E, Sato M, Takahashi T, Maehara M, Nakamura Y, Mitani G, Takagaki T, Hamahashi K, Watanabe M

2019

2018

Regenerative Therapy, 9, 24-31, 2018.

Cartilage repair and inhibition of the progression of cartilage degeneration after transplantation of allogeneic chondrocyte sheets in a nontraumatic early arthritis model.

Takatori N, Sato M, Toyoda E, Takahashi T, Okada E, Maehara M, Watanabe M

2018

Biochem Biophys Res Commun, 496(2), 429-435, 2018.

Quality assessment of cellular and tissue-based products using liquid chromatography-tandem mass spectrometry.

Tominaga A, Sato M, Takahashi T, Toyoda E, Toyoda K, Suzuki T, Takahashi M, Watanabe M, Okazaki K

2018

J Tissue Eng Regen Med, [Epub ahead of print] 2018.

Intra-articular administration of EP2 enhances the articular cartilage repair in a rabbit model.

Tani Y, Sato M, Yokoyama M, Yokoyama M, Takahashi T, Toyoda E, Okada E, Fujimura S, Maruki H, Kato Y, Yamato M, Okano T, Mochida J

2018

J Tissue Eng Regen Med, 12, 2067-2076, 2018.

Rabbit xenogeneic transplantation model for evaluating human chondrocyte sheets used in articular cartilage repair.

Takahashi T, Sato M, Toyoda E, Maehara M, Takizawa D, Maruki H, Tominaga A, Okada E, Okazaki K, Watanabe M

2018

2017

Inflamm Regen, 37:22, 2017.

Characterization of polydactyly-derived chondrocyte sheets versus adult chondrocyte sheets for articular cartilage repair.

Maehara M, Sato M, Toyoda E, Takahashi T, OkadaE, Kotoku T, Watanabe M

2017

J Biomed Mater Res B Appl Biomater, 105(8), 2592-2602, 2017.

Effects of a cell-free method using collagen vitrigel incorporating TGF-β1 on articular cartilage repair in a rabbit osteochondral defect model.

Maruki H, Sato M, Takezawa T, Tani Y, Yokoyama M, Takahashi T, Toyoda E, Okada E, Aoki S, Mochida J, Kato Y

2017

J Tissue Eng Regen Med, 11(12), 3437-3444, 2017.

The effects of using vitrified chondrocyte sheets on pain alleviation and articular cartilage repair.

Tani Y, Sato M, Maehara M, Nagashima H, Yokoyama M, Yokoyama M, Yamato M, Okano T, Mochida J

2017

J Tissue Eng Regen Med, 11(10), 2885-2894, 2017.

Characterization of layered chondrocyte sheets created in a co-culturesystem with synoviocytes in a hypoxic environment.

Kokubo M, Sato M, Yamato M, Mitani G, Uchiyama Y, Mochida J, Okano T

2017

J Tissue Eng Regen Med, 11(12), 3305-3312, 2017.

Platelet-activated serum might have a therapeutic effect on damaged articular cartilage.

Yokoyama M, Sato M, Tani Y, Yokoyama M, Kokubo M, Yamato M, Okano T, Mochida J

2017

2016

Stem Cell Research & Therapeutics, 1(1), 2016.

Application of chondrocyte sheet for cartilage regeneration.

Toyoda E, Sato M, Takahashi T, Maehara M, Mochida J

2016

Bone Joint Nerve, 6(3), 589-594, 2016.

膝OAに対するVEGF阻害剤の効果.

長井敏洋, 佐藤正人

2016

2015

Tissue Engineering part C Methods, 22(1), 59-68, 2015.

Assessment of the Safety of Chondrocyte Sheet Implantation for Cartilage Regeneration.

Yokoyama M, Sato M, Mitani G, Takagaki T, Yokoyama M, Okada E, Kokubo M, Yamato M, Ito N, Takaku Y, Murai K, Matoba R, Akutsu H, Yamato M, Okano T, Mochida J

2015

International Journal of Biological & Pharmaceutical Research 6, 351-358, 2015.

Interactions between jellyfish mucin and hyaluronan in human chondrocytes.

Takagaki T, Sato M, Kawake T, Baba T, Kihira K, Mochida J

2015

Arthritis Research & Therapy, 17:383, 2015.

Usefulness of using laser-induced photoacoustic measurement and 3.0 Tesla MRI to assess knee cartilage damage: a comparison study.

Ukai T , Sato M, Ishihara M , Yokoyama M, Takagaki T , Mitani G, Tani Y, Yamashita T, Imai Y, Mochida J

2015

BMC Musculoskeletal Disorders 16:35, 2015.

Diffusion tensor imaging can detect the early stages of cartilage damage: a comparison study.

Ukai T, Sato M, Yamashita T, Imai Y, Mitani G, Takagaki T, Serigano K, Mochida J

2015

2014

The Anatomical Record 297（1）, 36-43, 2014.

Articular cartilage regeneration using cell sheet technology.

Sato M, Yamato M, Hamahashi K, Okano T, Mochida J

2014

Biomaterials 35(7) 2199-2206, 2014.

In vivo cell tracking by bioluminescence imaging after transplantation of bioengineered cell sheets to the knee joint

Takaku Y, Murai K, Ukai T, Ito S, Kokubo M, Satoh M, Kobayashi E, Yamato M, Okano T, Takeuchi M, Mochida J, Sato M

2014

J Biomed Mater Res A, 102(9), 2927-2933, 2014.

Potential utility of cell sheets derived from the anterior cruciate ligament and synovium fabricated in temperature-responsive culture dishes.

Mitani G, Sato M, Yamato M, Kokubo M, Takagaki T, Ebihara G, Okano T, Mochida J

2014

Arthritis Research & Therapy 16(5): 427, 2014.

Bevacizumab, an anti-vascular endothelial growth factor antibody, inhibits osteoarthritis.

Nagai T, Sato M, Kobayashi M, Yokoyama M, Tani Y, Mochida J

2014

2013

BMC Biotechnol 13(58), 2013.

Development of a novel vitrification method for chondrocyte sheets.

Maehara M, Sato M, Watanabe M, Matsunari H, Kokubo M, Kanai T, Sato M, Matsumura K,Hyon SH, Yokoyama M, Mochida J, Nagashima H

2013

J Tissue Eng Regen Med, Published online 2013, 10(6), 486-495, 2013.

Characterization of chondrocyte sheets prepared using a co-culture method with temperature-responsive culture inserts.

Kokubo M, Sato M, Yamato M, Mitani G, Kutsuna T, Ebihara G, Okano T, Mochida J

2013

2012

J Tissue Eng Regen Med, 9(1), 24-30, 2012.

Studies of the humoral factors produced by layered chondrocyte sheets.

Hamahashi K, Sato M, Yamato M, Kokubo M, Mitani G, Ito S, Nagai T, Ebihara G, Kutsuna T, Okano T, Mochida J

2012

Biomaterials 33(21), 5278-5286, 2012.

Repair of articular cartilage defect with layered chondrocyte sheets and cultured synovial cells.

Ito S, Sato M, Yamato M, Mitani G, Kutsuna T, Nagai T, Ukai T, Kobayashi M, Kokubo M, Okano T, Mochida J

2012

Biomaterials 33(15), 3846-3851, 2012.

Cartilage repair in transplanted scaffold-free chondrocyte sheets using a minipig model.

Ebihara G, Sato M, Yamato M, Mitani G, Kutsuna T, Nagai T, Ito S, Ukai T, Kobayashi M, Kokubo M, Okano T, Mochida J

2012

Journal of Orthopaedic Research 30(12), 1915-1922, 2012.

MicroRNA-199a-3p, microRNA-193b, and microRNA-320c are correlated to aging and regulate human cartilage metabolism.

Ukai T, Sato M, Akutsu H, Umezawa A, Mochida J

2012

BMC Musculoskeletal Disorders 13(1):51, 2012.

Human telomerase reverse transcriptase and glucose-regulated protein 78 increase the life span of articular chondrocytes and their repair potential.

Sato M, Shin-ya K, Lee JI, Ishihara M, Nagai T, Kaneshiro N, Mitani G, Tahara H, Mochida J

2012

2011

European Cells and Materials 22, 275-290, 2011.

Transplantation of scaffold-free spheroids composed of synovium-derived cells and chondrocytes for the treatment of cartilage defects of the knee.

Lee JI, Sato M, Kim HW, Mochida J

2011

Lasers in Surgery and Medicine 43(5), 421-432, 2011.

A diagnostic system for articular cartilage using non-destructive pulsed laser irradiation.

Sato M, Ishihara M, Kikuchi M, Mochida J

2011

BMC Biotechnology, 11:19, 2011.

Measurement of diffusion in articular cartilage using fluorescence correlation spectroscopy.

Lee JI, Sato M, Ushida K, Mochida J

2011

2010

Arthritis Research & Therapy 12(5):R178, 2010.

Intravenous administration of anti-VEGF humanized monoclonal antibody bevacizumab improves articular cartilage repair.

Nagai T, Sato M, Kutsuna T, Kokubo M, Ebihara G, Ohta N, Mochida J

2010

Tissue Eng Part C Methods 16(3), 365-373, 2010.

Noninvasive evaluation of tissue-engineered cartilage with time-resolved laser-induced fluorescence spectroscopy.

Kutsuna T, Sato M, Ishihara M, Furukawa KS, Nagai T, Kikuchi M, Ushida T, Mochida J

2010

2009

BMC Biotechnology 9(17), 2009.

The properties of bioengineered chondrocyte sheets for cartilage regeneration.

Mitani G, Sato M, Lee JI, Kaneshiro N, Ishihara M, Ota N, Kokubo M, Sakai H, Kikuchi T, Mochida J

2009

BMC Biotechnology 9(98), 2009.

Jellyfish mucin may have potential disease-modifying effects on osteoarthritis.

Ohta N, Sato M, Ushida K, Kokubo M, Baba T, Taniguchi K, Urai M, Kihira K, Mochida J

2009

2008

Tissue Engineering Part A 14(7), 1225-1235, 2008.

Optimization of allograft implantation using scaffold-free chondrocyte plates.

Nagai T, Sato M, Furukawa KS, Kutsuna T, Ohta N, Ushida T, Mochida J

2008

Tissue Engineering Part A 14(7), 1183-1193, 2008.

Characteristics of a scaffold-free articular choncrocyte palte grown in rotational culture.

Nagai T, Furukawa KS, Sato M, Ushida T, Mochida J

2008

Medical and Biological Engineering and Computing 46(8), 735-743, 2008.

Recent technological advancements related to articular cartilage regeneration.

Sato M, Ishihara M, Furukawa K, Kaneshiro N, Nagai T, Mitani G, Kutsuna T, Ohta N, Kokubo M, Kikuchi T, Sakai H, Ushida T, Kikuchi, M, Mochida J

2008

2007

European Cells and Materials13, 87-92, 2007.

Cultured articular chondrocytes sheets for partial thickness cartilage defects utilizing temperature-responsive culture dishes.

Kaneshiro N, Sato M, Ishihara M, Mitani G, Sakai H, Kikuchi T, Mochida J

2007

Journal of biomedical materials research, Part B Applied biomaterials 83(1), 181-188, 2007.

Effects of growth factors on heparin-carrying polystyrene-coated atelocollagen scaffold for articular cartilage tissue engineering.

Sato M, Ishihara M, Ishihara M, Kaneshiro N, Mitani G, Nagai T, Kutsuna T, Asazuma T, Kikuchi M, Mochida J

2007

2006

Biochemical and Biophysical Research Communications 349(2), 723-731, 2006.

Bioengineered chondrocyte sheets may be potentially useful for the treatment of partial thickness defects of articular cartilage.

Kaneshiro N, Sato M, Ishihara M, Mitani G, Sakai H, Mochida J

2006

J Biomed Mater Res. Part B Appl Biomater 79(1), 25-34, 2006.

Tissue engineering of articular cartilage with autologous cultured adipose tissue-derived stromal cells using atelocollagen honeycomb-shaped scaffold with a membrane sealing in rabbits.

Masuoka K, Asazuma T, Hattori H, Yoshihara Y, Sato M, Matsumura K, Matsui T, Takase B, Nemoto K, Ishihara M

2006

Lasers in surgery and medicine 38(3), 249-255, 2006.

Development of a diagnostic system for osteoarthritis using a photoacoustic measurement method.

Ishihara M, Sato M, Kaneshiro N, Mitani G, Sato S, Mochida J, Kikuchi M

2006

2005

Journal of biomedical materials research. Part B, Applied biomaterials 75(1), 177-184, 2005.

Tissue engineering of articular cartilage using an allograft of cultured chondrocytes in a membrane-sealed atelocollagen honeycomb-shaped scaffold (ACHMS scaffold).

Masuoka K, Asazuma T, Ishihara M, Sato M, Hattori H, Ishihara M, Yoshihara Y, Matsui T, Takase B, Kikuchi M, Nemoto K

2005

2003

Journal of Biomedical Materials Research A 64 (2), 249-256, 2003.

An atelocollagen honeycomb- shaped scaffold with a membrane seal (ACHMS-scaffold) for the culture of annulus fibrosus cells from an intervertebral disc.

Sato M, Asazuma T, Ishihara M, Kikuchi T, Masuoka K, Ichimura S, Kikuchi M, Kurita A, Fujikawa K

2003

Spine 28(6), 548-553, 2003.

An experimental study of the regeneration of the intervertebral disc with an allograft of cultured annulus fibrosus cells using a tissue-engineering method.

Sato M, Asazuma T, Ishihara M, Ishihara M, Kikuchi T, Kikuchi M, Fujikawa K

2003

Medical Biological Engineering Computing 41(3), 365-371, 2003.

Tissue engineering of the intervertebral disc with　cultured annulus fibrosus cells using atelocollagen honeycomb-shaped scaffold with a membrane seal (ACHMS scaffold).

Sato M, Kikuchi M, Ishihara M, Asazuma T, Kikuchi T, Masuoka K, Hattori H, Fujikawa K

2003